Kikuoka H, Kouzaki H, Matsumoto K, et al. - Since clinical improvement was seen in only a half of Japanese cypress (JCy) pollinosis patients after treatment with sublingual immunotherapy (SLIT) with Japanese cedar (JCe) pollinosis, therefore, researchers focused on the immunological influence of SLIT for JCy pollinosis. From patients with JCe and JCy pollinosis who did and did not receive SLIT, peripheral blood mononuclear cells were incubated with Cry j 1, Cha o 1 and Cha o 3 antigens. They carried out basophil activation test (BAT). In the SLIT group, they found the inhibition of the generation of IL(interleukin)-5 and IL-17 provoked by antigens. They noted a rise in Cry j 1-specific generation of IL-10, as well as elevation in serum Cry j 1-specific IgE and -IgG4. However, no increase was seen in Cha o 1- or Cha o 3-specific generation of IL-10 and specific IgG4. Following SLIT, no reduction was evident in antigens-specific BAT. For patients with combined JCe and JCy pollinosis, there is a requirement of novel SLIT with JCe and JCy.