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Immunohistochemical detection of citrullinated histone H3‐positive neutrophils is useful for identifying active glomerular and interstitial lesions in antineutrophil cytoplasmic antibody‐associated vasculitis

Histopathology Feb 17, 2021

Kimura H, Mii A, Shoji J, et al. - In this study, the associations between infiltrating citrullinated histone H3 (H3Cit)‐positive neutrophils and disease specificity and activity in various glomerulonephritides were explored. In this study, 32 kidney biopsies with glomerulonephritides, including antineutrophil cytoplasmic antibody‐associated vasculitis (AAV), lupus nephritis, Henoch–Schönlein purpura nephritis, and post-streptococcal acute glomerulonephritis were selected. Researchers analyzed the presence of H3Cit in infiltrating neutrophils and their relationship with necrotizing, crescentic lesions, and tubulointerstitial lesions. H3Cit immunostaining was found to be a beneficial tool for distinguishing activated neutrophils. The findings exhibited that in AAV, the frequency of H3Cit+ neutrophils is not only a disease‐specific marker but also a potential marker for disease activity.

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