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Immunohistochemical detection of citrullinated histone H3‐positive neutrophils is useful for identifying active glomerular and interstitial lesions in antineutrophil cytoplasmic antibody‐associated vasculitis

Histopathology Nov 06, 2020

Kimura H, Mii A, Shoji J, et al. - In this study, the association between infiltrating citrullinated histone H3 (H3Cit)‐positive neutrophils and disease specificity and activity were explored in various glomerulonephritides. Researchers selected 32 kidney biopsies with glomerulonephritides, including AAV, lupus nephritis (LN), Henoch–Schönlein purpura nephritis, and post-streptococcal acute glomerulonephritis (PSAGN). They analyzed the presence of H3Cit in infiltrating neutrophils and their relationship with necrotizing, crescentic lesions, and tubulointerstitial lesions. H3Cit immunostaining was found to be a beneficial tool for distinguishing activated neutrophils. The data revealed that the frequency of H3Cit+ neutrophils is not only a disease‐specific marker but also a potential marker for disease activity in AAV.

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