Bartel S, La Grutta S, Cilluffo G, et al. - Researchers determined the impact of asthma development on the miRNA content of extracellular vesicles (EV) secreted by primary normal human bronchial epithelial cells (NHBE). They cultured NHBE cells at air-liquid interface and asthma-like phenotype was induced by treating these cells with interleukin 13. Nanoparticle Tracking Analysis, Transmission Electron Microscopy and Western Blot were used to characterize EV isolations by precipitation from basal culture medium or apical surface wash. Using a RT-qPCR-based profiling, they identified EV-associated miRNAs. Using size exclusion chromatography, EVs were isolated from nasal lavages of children with mild-to-moderate (n = 8) or severe asthma (n = 9), and healthy controls (n = 9). In these EVs, they confirmed significant candidates. In this study, the possible involvement of secretion of miRNAs in EVs from the airway epithelium, especially miR-34a, miR-92b and miR-210, in the early development of a Th2 response in the airways and asthma was suggested.