Khosropour CM, Jensen JS, Soge OO, et al. - Researchers investigated the prevalence of rectal Mycoplasma genitalium (MG), rectal MG/Chlamydia trachomatis (CT)/Neisseria gonorrhoeae (GC) coinfection, and MG antimicrobial resistance patterns among women. Fifty women at high risk for CT were recruited from Seattle's municipal sexually transmitted disease clinic from 2017 to 2018. CT/GC nucleic acid amplification testing on self-collected vaginal and rectal specimens revealed 13 (26%) cases positive for MG, including 10 (20%) with vaginal MG and 11 (22%) with rectal MG, and 8 (62%) cases with concurrent vaginal/rectal MG. Observations suggest that macrolide-resistant vaginal and rectal MG are highly prevalent among a population of women at high risk for CT. No vaginal or rectal MG-positive specimens exhibited  ParC quinolone resistance-associated mutations (QRAMs), which were previously linked with quinolone failure. Azithromycin was administered to five MG-infected women for vaginal CT, 4 of whom had a MG with macrolide resistance-mediating mutations (MRM) detected in their vaginal and/or rectal specimens. This work revealed how the use of antimicrobials designed to treat an identified infection—in this case, CT—could affect treatment outcomes and antimicrobial susceptibility in other unidentified infections. .