Mestres E, García-Jiménez M, Casals A, et al. - Researchers aimed at determining lab-related factors that affect the culture system’s capacity to maintain a stable osmolality during human embryo culture. They performed incubation of dishes in parallel to analyze osmolality during culture between groups: synthetic oviductal medium enriched with potassium versus human tubal fluid medium; protein vs no protein supplementation; dry vs humid atmosphere; high vs low volume of mineral oil. In addition, they compared media evaporation between six models of time-lapse dishes with distinct designs, cultured in a joint incubator. Retesting was performed on two of them in their corresponding incubator to analyze the dish-incubator fit. Per findings, following are important parameters that can affect media evaporation and consequently osmolality during culture: incubator humidity, the volume of mineral oil, the type of culture media and the design of time-lapse dishes.