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Exploring the phenotype and genotype of multi-drug resistant Klebsiella pneumoniae harbouring blaCTX-M group extended-spectrum β-lactamases recovered from paediatric clinical cases in Shenzhen, China

Annals of Clinical Microbiology and Antimicrobials Nov 11, 2019

Patil S, et al. - Researchers assessed carbapenemase and extended-spectrum β-lactamase producing K. pneumoniae isolated from pediatric patients in Shenzhen, China for their drug resistance profile and molecular characterization. This study, for the first time, described the occurrence and spread of extended β-lactamase K. pneumoniae ST20 and ST2407, in this region and particularly in paediatric population. From 2014 and 2015, a total of 31 isolates of multi-drug resistant K. pneumoniae were collected. All of these isolates 100% were noted to be resistant to ceftazidime, ertapenem, ampicillin, cefazolin and ampicillin-sulbactam followed by ceftriaxone 94% (n = 29), aztreonam 89% (n = 26), cefepime 84% (n = 26), nitrofurantoin 75% (n = 24), piperacillin 52% (n = 16), and levofloxacin 49% (n = 15). Of the 31 β-lactamase gene coding isolates, about 100% (n = 31) exhibited mainly blaCTX-M, followed by blaKPC in 71% (n = 22), blaSHV 61% (n = 19), blaNDM 25% (n = 8), blaCYM 13% (n = 4), blaOXA-48 9% (n = 3), blaGES 9% (n = 3) and blaTEM 6% (n = 2). They identified 17 distinct sequences type with ST20 being mostly identified 16% (n = 5). As per plasmid replicon typing, the presence of IncFIS type was highest in all isolates as 61% (n = 19), followed by IncFIB 23% (n = 7), IncFIA and IncFIC 16% (n = 5) each.
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