Yang JH, et al. - In this investigation involving 30 patients, researchers identified the impact of mircoRNA-335 (miR-335) methylation on metastasis of nasopharyngeal carcinoma. They applied RT-PCR and methylation-specific PCR to identify the expression levels of miR-335 and miR-335 methylation in nasopharyngeal carcinoma tissues. Compared with those with promoter unmethylation, the levels of miR-335 expression were significantly lower in nasopharyngeal cancer tissues with promoter methylation. In 14 out of 30 nasopharyngeal cancer tissues, the levels of miR-335 gene promoter methylation were higher. Moreover, compared with patients without cervical lymph node metastasis those with cervical lymph node metastasis had higher methylation rate in miR-335 promoter. Findings suggested an association of miR-335 methylation expression with nasopharyngeal carcinoma metastasis.