Na Takuathung M, et al. – This study probed the therapeutic potential of Wannachawee Recipe (WCR) on antiproliferation and anti–inflammatory effects in HaCaT human keratinocytes, in the treatment of psoriasis. The findings successfully determined WCR as an alternative therapy yielded from natural products. It exhibited potent anti–inflammatory effects, without the presence of cytotoxicity and did not induce cell cycle arrest, on the HaCaT cells. In order to disclose all aspects of antipsoriatic properties of WCR to affirm WCR as being effective and safe, advanced research was necessitated.

Methods

• Wannachawee Recipe (WCR) was successfully used used as Thai folk remedy for psoriasis patients.
• Nevertheless, there was a lack of preclinical evidence defining how WCR works.
• The mechanisms for its antiproliferation and anti-inflammatory effects in HaCaT cells were defined here.

Results

• WCR inhibited the growth and viability of HaCaT cells in a concentration-dependent manner.
• This was illustrated via the cytotoxicity and antiproliferation results from SRB and CCK-8 assays.
• The distribution of cell cycle phases ascertained via flow cytometry exhibited that WCR did not interrupt cell cycle progression.
• RT-qPCR disclosed that WCR notably decreased the mRNA expression of IL-1β, IL-6, IL-8, IL-17A, IL-22, IL-23, and TNF-α but induced IL-10 expression in TNF-α- and IFN-γ-induced HaCaT cells.
• At the protein level determined by ELISA, WCR considerably reduced the secretion of IL-17A, IL-22, and IL-23.