Murugesu S, et al. - The purpose of this trial was to assess the effect if any, of calcium ionophore as a technique of artificial oocyte activation (AOA) on pregnancy outcomes and fertilization rates. A substantial improvement was yielded in fertilization, cleavage, blastulation, and implantation rates, as well as overall pregnancy and live-birth rates with AOA with calcium ionophore treatment after intracytoplasmic sperm injection (ICSI). It illustrated a strong effect of calcium ionophore use. This data was reassuring and promising, particularly for couples for whom ICSI alone led to poor fertilization rates.