Takashima Y, Yoshimura T, Kano Y, et al. - Via the first study for the expression profile of N-oligosaccharides on methotrexate (MTX)-resistant primary CNS lymphoma-derived cells HKBML and TK, and tumor tissues resected from patients with CNS lymphoma, experts examined expression patterns of N-linked oligosaccharides of glycoproteins in cells derived from CNS lymphomas and clinical specimens. MTX-resistant cells derived from HKBML and TK as CNS lymphoma, and RAJI as non-CNS lymphoma were generated and N-linked oligosaccharide structures in these cells and other non-CNS lymphoma-derived cells including A4/FUK, OYB, and HBL1 were ascertained. Major components of the total oligosaccharides were high-mannose type N-glycans, whose level rose in MTX-resistant HKBML and TK though reduced in MTX-resistant RAJI. Sialylated biantennary galactosylated N-glycans with α1,6-fucosylation, A2G2F, and A2G2FB from HKBML, TK, and RAJI was also discovered. Sialylated A4G4F was particularly isolated from RAJI. Notwithstanding, the ratios of these sialylated N-glycans slightly reduced against MTX-resistant in comparison with non-resistant cells. Interestingly, nearly all complex-type oligosaccharides were α2,6-sialylated. Thus, these conclusions propose a likelihood that the differential expression of high-mannose types and sialylated A2G2F, A2G2FB, and A4G4F on the surface of CNS lymphomas may give a sign for targets for diagnoses and treatments of the oligosaccharide type-specific lymphomas.