Basile U, et al. - Authors probed into the presence of IgG rheumatoid factor (RF) and anti-nuclear antibodies (ANA) in cryoprecipitates of subjects with Type III and Type II mixed cryoglobulinemia (MC) with the intention of comprehending the biochemical patterns related to different types of MC to a greater degree, assessed via immunofixation for IgG3 and through ELISA for IgG RF. Statistically varied ANA patterns were revealed between type II and type III MC after stratification according to MC type. Findings demonstrated markedly higher IgG3 levels and IgG-RF positivity in type III cryoprecipitate, alongside a higher positivity of IgG3 and a notable variation between the liver fibrosis stage, ANA and IgG-RF in the cryoprecipitate. A combination of biochemical markers and autoantibodies associated to mixed cryoglobulinemia were demonstrated.