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Diagnostic value of a nested polymerase chain reaction for diagnosing cutaneous sporotrichosis from paraffin-embedded skin tissue

Mycoses Sep 22, 2019

Hayashi S, Kaminaga T, Baba A, et al. - Researchers conducted this investigation to examine the diagnostic value of a molecular method for diagnosing cutaneous sporotrichosis from formalin-fixed and paraffin-embedded tissues. They collected 52 cases of cutaneous sporotrichosis from biopsied specimens with positive diagnoses via culture test over a 30-year period. Of the 52 patients who tested positive on the culture test, PCR identified all cutaneous diseases. Fifty-eight tested negative by PCR of the 59 patients in the control group. Findings suggested that the particular PCR assay used seems to be a helpful tool for the prompt and accurate diagnosis of sporotrichosis. Using this technique, cutaneous sporotrichosis could be diagnosed in patients suspected of cutaneous sporotrichosis but who tested negative on culture, and in patients with pathologically suspected cutaneous sporotrichosis for whom the culture test was not performed.

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