Moon A, et al. - The purpose of this research was to develop CD3 cell quantitation algorithms for renal allograft biopsy rejection assessment, with the aid of an open source image analysis software. This approach displayed salient correlations with established methods of CD3 quantitation. Such analysis techniques were found to be promising and highly customizable. They yielded a form of on-slide “flow cytometry” which possibly facilitated additional diagnostic accuracy in tissue-based assessments.