Cheloni R, et al. - Given that recent developments in optical coherence tomography (OCT) technology enable direct enface visualisation of retinal nerve fibre bundle (RNFB) loss in glaucoma, however, the optimum depth at which to visualise RNFBs across the retina is not known, researchers sought to determine the range of depths and optimum depth at which RNFBs can be visualised across the retina in healthy eyes. The central ± 25° retina of 10 healthy eyes from 10 people aged 57 to 75 years (median 68.5 years) were imaged with spectral domain OCT. RNFB visibility exhibited good agreement between graders and excellent repeatability. Retinal nerve fibre layer thickness (RNFLT) was highly associated with a depth range of visible RNFBs.The depth range for visible RNFBs varies significantly, consistent with RNFLT, across the healthy retina. Slab properties can account for variations across retinal locations and between individual eyes to extract all RNFB information consistently across the retina.