Iioka H, et al. - In this work, researchers sought to illustrate that tumor invasion and metastasis of human colon adenocarcinoma cells are strongly promoted by epithelial cell polarity regulator Crumbs3 (Crb3), a mammalian homolog within the Drosophila Crb gene family. Invasive front and metastatic foci of colonic adenocarcinoma of the patient tissues displayed the strong expression of Crb3 indicating its centrality to tumor migration. Accordingly, the CRISPR/CAS9 system generated two different Crb3-knockout (KO) lines, Crb3-KO (Crb3 -/-) DLD-1 and Crb3-KO WiDr from human colonic adenocarcinomas. As per proteome analysis of Crb3-coimmunopreciptated proteins, there were different respective FGFR isotypes that were specifically bound to Crb3a through their intracellular domain in DLD-1 vs WiDr. Primary and metastatic colorectal cancer patient tissues consistently showed correlative expression between Crb3 and FGFR1. Through specific interaction to FGFR1 on colon cancer cells, Crb3 was identified accelerating cell migration, namely invasion and metastasis of human colon cancers.