Santiago T, et al. - A pilot cohort of 78 individuals with neuroblastoma (NB), including 34 of Middle Eastern descent (MED) who had a greater prevalence of MYCN gene amplification and 44 of North American descent (NAD), nine of whom had MYCN amplification, was assessed with immunohistochemistry (IHC) for MYCN protein in order to examine the feasibility of using IHC to identify MYCN protein expression in lieu of fluorescent in situ hybridization (FISH), an approach possibly beneficial in areas with insufficient resources. An outstanding overall correlation among MYCN gene status by FISH and MYCN protein expression by IHC was validated. In a limited-resource setting, MYCN IHC in NB with reflexing to FISH in equivocal cases was found out to be conceivably beneficial. Moreover, assessment of efficacy via a larger cohort and optimization to conduct MYCN IHC manually is required.