Heppt MV, Clanner-Engelshofen BM, Marsela E, et al. - In the present study, the researchers sought to assess the phototoxicity of 17 distinct agents and ascertain if BRAF inhibitors (BRAFi)-induced phototoxicity may be alleviated by antioxidants. For this investigation, the ultraviolet (UV) light absorbance of 17 BRAFi was determined. Their phototoxic potential was examined independently with a reactive oxygen species and the 3T3 neutral red uptake (NRU) assay in vitro. According to results, vemurafenib was observed with the highest cumulative absorbance for both UVA and UVB. The formation of singlet oxygen and superoxide anion and the phototoxicity found in the NRU 3T3 assay were significantly associated. The study confirms that most BRAF inhibitors, predominantly after exposure to UVA, showed considerable phototoxic potential. Glutathione can help to treat and prevent vemurafenib-induced phototoxicity.