Zhang Y, et al. - Researchers investigated the clinical significance as well as potential biological functions of metadherin/astrocyte elevated gene-1 (MTDH/AEG-1) in bladder urothelial carcinoma (BUC). Using the Cancer Genome Atlas (TCGA) database and immunohistochemistry, they measured expression of MTDH/AEG-1in BUC. In addition, the expression and diagnostic value of MTDH/AEG-1 were determined via a meta-analysis. In vitro analysis of the possible link between MTDH/AEG-1 expression and the viability, proliferation, and apoptosis in BUC cell lines (T24, HT1376, and RT4) was also carried out, using viability, MTS, colony formation, and caspase-3/7 assays, as well as Hoechst 33342 and propidium iodide (PI) double staining. The TCGA and immunohistochemistry results revealed a significantly higher MTDH/AEG-1 expression in BUC tissues vs in normal bladder tissues, and meta-analysis outcomes verified these findings. Overall, a presumption was formulated that MTDH/AEG-1 could play essential roles in BUC, especially in cell growth and apoptosis, via the insulin signaling pathway.”