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Circulating Ara h 6 as a marker of peanut protein absorption in tolerant and allergic humans following ingestion of peanut‐containing foods

Clinical & Experimental Allergy Jul 16, 2020

Bernard H, Turner PJ, Ah‐Leung S, et al. - Researchers sought to construct a protocol enabling the identification of the major peanut allergen, Ara h 6, in the bloodstream after ingestion of low amounts of peanut, and to compare Ara h 6 bioaccessibility by food matrix. They further evaluated for disparities in absorption in healthy vs peanut‐allergic volunteers. They developed a blood pretreatment integrating acidic shock and thermal treatment, this was followed by application of this protocol to blood samples obtained from human volunteers at several timepoints after ingestion of rising levels of peanut incurred in different food matrices (cookies, peanut butter, chocolate dessert). Findings revealed the dependence of kinetics as well as intensity of Ara h 6 passage in bloodstream on both individual and food matrix. Peanut‐allergic patients seemed to show higher absorption rate, the clinical value of which justifies further assessment.

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