Johansson MW, et al. - Researchers used flow cytometry to analyze Siglec-8 levels (a potential therapeutic target in eosinophilic asthma) on eosinophils and basophils recruited during lung inflammation. They used confocal microscopy to compare Siglec-8 distribution on bronchoalveolar lavage and blood eosinophils in human subjects with mild allergic asthma. They found the resemblance between overall levels of Siglec-8 expression on airway eosinophils relative to those on blood eosinophils, after 48 h after segmental allergen challenge, but a patchier distribution, a pattern consistent with activation, was seen. Siglec-8, when therapeutically targeted, could potentially influence blood as well as lung eosinophils, which may be related to an improved outcome in eosinophilic lung diseases.