Singh T, et al. - Researchers examined 120 E. coli isolates (both diarrheagenic and non-pathogenic) recovered from fresh stool samples collected from children aged less than 5 years from Delhi, India, for analysis of antibiotic resistant genes and single nucleotide polymorphism (SNP) in gyrA and parC genes in quinolone resistance-determining region (QRDR). In phylogenetic analysis, clonal diversity and phylogenetic relationships among the E. coli isolates were identified. Mutations in gyrA and parC genes in QRDR were identified in the SNP analysis. Almost half (47.5%) of the isolates across the diseased and healthy samples had the sul1 gene, responsible for sulfonamide resistance. E. coli isolates from healthy children displayed antibiotic resistance genes suggesting the development, dissemination and carriage of antibiotic resistance in their gut. Findings support the implementation of active surveillance and stewardship programs to improve appropriate antibiotic use and reducing further danger.