Guazzaroni M, Ferrari D, Lamacchia F, et al. - This study was designed to analyze elastosonography evaluation of both eyes of 40 glaucomatous patients and 40 healthy individuals. Shear wave elastography with a one-dimensional-array probe was used to capture two-dimensional images in order to study the optic nerve near the papilla, chorioretinal complex, lateral rectus muscle, and periorbital fat tissue. Moreover, they applied a two-dimensional array probe to capture three-dimensional images to study the optic nerve in toto with multilevel sampling. Then, qualitative and quantitative data (“absolute” stiffness values) were obtained. They found statistically significant differences, between glaucomatous individuals and healthy individuals, in “absolute” stiffness values in the two-dimensional analysis at the emergence of the optic nerve and chorioretinal complex, and in the three-dimensional analysis at the emergence of the optic nerve (level I°). The results suggest the use of elastosonography to verify the existence of pathological alterations in the mechanical and elastic properties of peri-ocular structures and their variations as a complementary tool in the diagnosis of glaucoma and for follow-up during treatment.