Piao L, et al. - Researchers investigated the role of B7H4 as a cancer stem cell (CSC) regulator in esophageal squamous cell carcinoma (ESCC) and its expression levels compared to other stemness genes in ESCC. In 156 paraffin embedded ESCC tissue samples, they evaluated the expression of B7H4 and cancer stemness proteins using immunohistochemistry, as well as in ESCC cell lines using western blotting and immunofluorescence imaging. It was observed that B7H4 expression was positively linked with the expression of cell cycle regulators such as cyclin D1, p27, and PI3K/Akt/NF-B signaling proteins. For the prognostic evaluation of ESCC patients, B7H4 could be a novel cancer stem cell marker. It could also be a potential therapeutic target against ESCC.