Matte I, et al. - Researchers determined if ovarian cancer (OC) ascites stimulate the expression and release of MUC16 by human peritoneal mesothelial cells (HPMCs), given malignant ascites is a pro-inflammatory environment. This study included women operated for conditions other than cancer, from peritoneal lavages of whom, HPMCs were isolated. Depending on the experiments, they used immunoblot, immunofluorescence or immunohistochemistry to determine MUC16 protein expression. Using EIA, they measured the release of MUC16 from the cell surface and using droplet Digital PCR, MUC16 mRNA expression was determined. Findings highlighted the importance of mesothelial cells as a source of MUC16 in the context of ovarian cancer. Malignant ascites was identified as a strong modulator of MUC16 expression in HPMCs. For upregulation of MUC16, the Akt pathway was recognized as a driving factor.