Pina-Vaz C, et al. - Researchers investigated the activity of three drugs with local anesthetic activity (namely benzydamine, lidocaine, and bupivacaine) against Candida albicans and non-albicans strains. Further, they ascertained their mechanism of activity. Determination of the minimal inhibitory concentration (MIC) for 20 Candida strains (18 clinical isolates and two American Type Culture Collection strains) revealed the MIC ranging from 12.5–50.0 µg/mL, 5.0–40.0 mg/mL, and 2.5–10.0 mg/mL for benzydamine, lidocaine, and bupivacaine, respectively. The fluorescent probe FUN-1 allowed the detection of the inhibitory activity of these concentrations after incubation for 60 minutes. In this work, 0.2, 50, and 30 mg/mL of benzydamine, lidocaine, and bupivacaine, respectively, exhibited a very fast fungicidal activity. Fungistatic activity of the tested drugs was observed at lower concentrations due to yeast metabolic impairment, while at higher concentrations they are fungicidal, due to direct damage to the cytoplasmic membrane.