Mamishi S, Mahmoudi S, Naserzadeh N, et al. - Researchers examined a number of gram-negative bacteria (GNB) causing hospital-acquired infection (HAI) in patients who were referred to Children’s Medical Center for antibiotic resistance; further, they performed genotyping of these GNB. During the 6-month period, they isolated 142 (1.1%) gram-negative bacterial strains. Isolation of most of these was done from blood (63%) followed by wounds (13.7%). From intensive care units, isolation of the greatest proportion of strains was observed (51%). The analysis revealed a low sensitivity of Acinetobacter baumannii to common antibiotics and high resistance of Enterobacter spp. to cefotaxime (100%). For Escherichia coli strain, amikacin (84%) and gentamycin (81%) were the most efficient antibiotics. However, resistance to trimethoprim-sulfamethoxazole was reported in only 12.5% of Serratia marcescens strains. The analysis of random amplified polymorphic DNA (RAPD)-typing revealed the presence of one clone in A. baumannii and E. coli and two clones in Klebsiella pneumoniae, whereas completely different trend was observed in Pseudomonas aeruginosa strains and Enterobacter spp. Results indicate that an outbreak in the Children’s Medical Center is possible. They recommend paying high attention to the basic methods of preventing infection as there is a possibility of transmission of an infection from one patient to another.