Grenga I, et al. - The effect of M7824 on invasive urothelial carcinoma cell lines was evaluated in this research. The immunomodulatory properties of M7824 on both tumor cell phenotype and immune-mediated lysis were illustrated. M7824 induced immunogenic modulation of urothelial carcinoma cell lines, rendering them more susceptible to immune-mediated recognition and lysis compared to anti-PD-L1 or M7824mut. Hence, the relevance of the dual blockade of PD-L1 and TGFβ in urothelial carcinoma cell lines and thus support the rationale were displayed for prospective trials of M7824 in patients with urothelial cancer.