Rietveld A, Wienke J, Visser E, et al. - In the present study, the researchers sought to evaluate anti‐cytosolic 5′‐nucleotidase 1A (cN‐1A/NTC51A) autoantibodies in children with juvenile dermatomyositis (JDM) and healthy controls, utilizing three different methods of antibody detection, as well as verification of the results in an independent cohort. Anti‐cN‐1A reactivity was evaluated in 34 Dutch JDM patients and 20 healthy juvenile controls through a commercially available full‐length cN‐1A ELISA, a synthetic peptide ELISA and by immunoblotting using a lysate from cN‐1A expressing HEK‐293 cells. According to findings, JDM patients and young healthy individuals do not exhibit anti‐cN‐1A reactivity as evaluated by various antibody detection techniques.