Canbek U, Dibek E, Akgun U, et al. - In this study, the biochemical, biological, and advanced proteomic analysis of the drainage fluid collected from prolonged wound drainage (PWD) patients following hip hemiarthroplasty (HA) was conducted. Researchers prospectively examined data of 28 patients who developed PWD after HA. They conducted biological and proteomic analyzes of both drainage fluid and serum. They further assessed similarities and differences in terms of protein concentrations, protein identities. The analysis enrolled 16 male patients with PWD (61.1%), 12 female patients (38.9%), and the mean age of all patients was 79.64 ± 8.44 (65-95). The results exhibited that when we carried out further proteomic analysis, PWD contains lymph-specific proteins, despite the similarity in serum and transudative PWD fluid in terms of biochemical content. Unlike PWD, they did not ascertain these proteins in serum. The results recognized that PWD fluid with abundant proteins may also serve an appropriate environment for the growth of microorganisms.