Caiado RR, et al. - Among 25 patients with chronic idiopathic macular holes (MHs), the applicability of the acai dye at a 25% concentration was tested to detect the posterior hyaloids and internal limiting membranes (ILMs) during pars plana vitrectomy (PPV). Subjects with glaucoma, previous significant ocular conditions, and previous ocular surgeries except uncomplicated cataract were excluded from the study. Ten surgeons carried out 23-gauge four-port PPV, phacoemulsification, posterior hyaloid detachment, ILM peeling guided by dye staining, and perfluoropropane injection. The dye’s staining abilities were assessed using a questionnaire. Compared to preoperatively, significantly improved final best-corrected visual acuity was obtained at 180 days postoperatively. In humans, the detection of posterior hyaloids and ILMs during PPVs was enabled by the use of acai dye at a 25% concentration. Dye was unlikely to cause toxicity.