Brink HV, et al. - Researchers conducted this method comparison study with the aim to ascertain the level of agreement across evaluations of follicle number per ovary (FNPO) and classifying of polycystic ovarian morphology (PCOM; FNPO ≥ 25), employing various real-time (RT) and off-line two-dimensional (2D) and three-dimensional (3D) ultrasonographic methods. They analyzed 32 ovaries in 16 women with and without PCOM employing eight ultrasonographic methods: 2D-Grid (reference method), 2D-RT, 2D-RT with Grid, multiplanar view (MPV), MPV-RT, tomographic ultrasound imaging (TUI), TUI-RT, and semiautomated volume calculation (SonoAVC). Ultrasonographic methods varied in FNPO limiting their interchangeable use, particularly when a precise metric is required. 2D-RT may be problematic because of its propensity to misclassify PCOM. For obtaining FNPO and classifying PCOM, they suggest possible utility of 2D-RT with Grid and MPV-RT as clinically feasible alternatives. The relevance of PCOM in women’s health will be clarified by efforts to lower variation in FNPO.