Pulmospheres, three dimensional multicellular spheroids composed of lung cells from individual patients, were shown to be effective in predicting the efficacy of medications for idiopathic pulmonary fibrosis, according to findings from University of Alabama at Birmingham scientists presented in JCI Insight journal.

Pulmospheres are tiny spheres — about one millimeter in diameter — which contain all the various cell types found in a human lung and are grown from tissue obtained from a surgical lung biopsy. Pulmospheres give researchers a 3D model to study various aspects of cell biology and disease mechanisms.

“Our results suggest that pulmospheres simulate the microenvironment in the lung and serve as a personalized and predictive model for assessing responsiveness to antifibrotic drugs in patients with IPF,” said Veena Antony, MD, professor in the Division of Pulmonary, Allergy and Critical Care Medicine within the Department of Medicine, and primary investigator of the study.

The UAB research team grew pulmospheres from 20 patients with idiopathic pulmonary fibrosis — a devastating lung disease — and nine control patients. They then examined whether the pulmospheres reacted to one of two commonly used medications for IPF.

The pulmospheres were grown to useable size in about 24 hours following the biopsy, then exposed to the two medications, pirfenidone and nintedanib. Within about 16 hours, researchers were able to observe if the spheres responded favorably to one, both or neither of the medications.

“This is a wonderful example of precision medicine,” said Victor Thannickal, MD, professor and director of the Division of Pulmonary, Allergy and Critical Care Medicine and a study co–author. “Using pulmospheres derived from a patient’s own cells may allow clinicians to tailor specific drugs to an individual patient without exposing that patient to potential side–effects or harm from treatments that are unlikely to be effective.”

Of the 20 subjects enrolled in this study, three patient’s pulmospheres responded only to nintedanib and the pulmospheres of four patient’s responded only to pirfenidone. Eleven patient’s pulmospheres responded to both drugs and two patient’s pulmospheres did not respond to either drug.

Researchers confirmed the findings by following the patients over time, establishing that the response predicted by the pulmospheres was clinically observed in the patients.Antony says there is a critical need for a better predictive model for IPF. Animal models of the disease are disappointing, and more traditional two dimensional cellular models are insufficient.

“The lungs are three dimensional organs and to truly understand the dynamics of IPF medications on the disease we require a 3D model, one that contains all the cell types found in a lung and that is able to function as a microcosm of the lung,” Antony said. “Many drugs have shown promise in pre–clinical studies, only to fail in subsequent clinical trials. Three dimensional modeling might change that.”

Antony says pulmospheres were developed in cancer research as a means of targeting potential drugs. A hallmark of IPF is an aggressive invasion by cells known as myofibroblasts, which mirrors the invasive phenotype of malignant cells, giving researchers reason to think that pulmospheres might work in IPF. The UAB trial was the first to study pulmospheres in IPF.

Antony also hopes that modern drug discovery techniques, using high throughput screening technology to quickly screen numerous compounds for disease–modifying properties, will be enhanced by the use of pulmospheres.

“There are many potential therapeutic agents for IPF in the discovery pipeline now, and this technique might prove to be a very effective way to determining which are the most promising,” she said.