The diverse 'coats' which protect a deadly microbe from our immune cells are generated by a 'hotspot' of rapidly evolving genes, a study has found.

Vaccines introduced over the last 20 years have been successful in reducing death rates by targeting the most lethal subtypes or ‘serotypes’ of pneumococcal bacteria – a handful of the estimated almost 100 identified. However, other strains of the bacteria not covered by the vaccines are becoming more common.

Now, a team led by researchers at Imperial College London has uncovered an ‘evolutionary hotspot’ in the bacterium’s genome which helps it to evade the human immune system by tweaking its outermost layer.

Their findings suggest the rapid rate of change in this region of DNA is a driving force behind the emergence of new serotypes – a process which could pose a challenge to future vaccine developments.

The researchers say that while there is strong evidence that vaccines provide good protection against pneumococcal pneumonia, a better understanding of how bacterial variation occurs at the genetic level could help scientists to stay one step ahead of the lethal bugs.

A key element which sets serotypes apart from one another is their capsule – the coat of densely–packed layers of sugars which envelopes the bacteria and acts like armour, protecting them from being identified and attacked by the body’s patrolling immune cells.

In the study, published in the journal Molecular Biology and Evolution, researchers from Imperial looked at genetic data for thousands of pneumococcal bacteria samples from around the world. A total of 4,519 samples from 29 countries were studied, with a focus on how variation in their capsules has evolved over time.

Analysis revealed that one region of their DNA in particular, called the cps locus, has been accumulating changes at a high rate relative to much of the rest of the genome. This evolutionary hotspot houses the genes responsible for the bacteria’s sugary coat of armour and accumulates mutations and new sections of DNA at more than twice the rate of the rest of the genome.

Comparing DNA from different strains revealed a number of common shared genetic elements – evidence that the bugs have swapped DNA in the past, in a process called horizontal gene transfer.

What’s more, they found that beyond trading DNA with related pneumococcal strains, there was evidence the bugs had picked up DNA from different species of Streptococcus, which could be a potential pathway for generating new drug–resistant bugs.

The two widely used vaccines for pneumococcal bacteria work by flagging sugars found in the bacterial capsules to cells of the immune system, so that if the immune cells recognises them in future they can mount a stronger defence. The evolutionary hotspot in the genome has the potential to give rise to new sugar combinations and capsules which could potentially evade these vaccines.

However, the researchers say that the genetic data only form a part of the story. Factors such as environment, public health and drug development all play a role in new the emergence of new strains. The researchers are confident that immunisation is still the best weapon in the fight against the bugs.