A Japanese research group has successfully grafted human iPS cell-derived inner ear cells that express human-derived proteins into the inner ears of embryonic mice, Kumamoto University announced on its website.

In human hereditary hearing loss, deafness has already occurred by birth. It is therefore considered that treatment during the fetal stage would be most effective. The GJB6 gene encodes the CONNEXIN 30 protein, which is essential in hearing development, and a deficiency of the gene results in the second most common disease among cases of hereditary hearing loss that have no other disease in the inner ear.

In previous work, Dr. Ryosei Minoda, head of the Department of Otolaryngology at Kumamoto General Hospital, reported that hearing loss was restored by gene therapy to the inner ear of CONNEXIN 30-deficient fetal mice. The current research team, which includes Minoda and Dr. Hiroki Takeda of Kumamoto University, and several researchers from Keio University, have successfully grafted human iPS-derived cells into the inner ear of embryonic mice.

First, the research team succeeded in efficiently inducing inner ear cells expressing inner-ear specific proteins, such as CONNEXIN 26, CONNEXIN 30, and PENDRIN, from human iPS cells. They then transplanted progenitors of the inner ear cells into the inner ear of embryonic normal and CONNEXIN 30 knockout mice using glass tubes with optimized tip sizes. The transplanted cells grafted to various sites throughout the inner ear in both groups. However, the CONNEXIN 30knockout mice had more grafted cells than the normal mice, and some of the grafted cells were found to express CONNEXIN 30.

According to the announcement, the fact that CONNEXIN 30 knockout mice had a higher number of grafted cells than normal mice, and that some of the grafted cells expressed CONNEXIN 30 is a very important finding when considering cell transplantation as a treatment for hereditary hearing loss caused by CONNEXIN deficiency. Cell transplantation can compensate for missing CONNEXIN and may improve hearing loss by adding properly functioning CONNEXIN proteins. Future work will attempt to increase the number of grafted cells and improve hearing ability.

Furthermore, this study revealed that cells derived from humans can be grafted into the heterozygous inner ear of mouse embryos. This adds the possibility of in-vivo experiments on therapeutic effects using human-derived cells in the mouse embryo. It is thought that this line of research can greatly contribute to the development of a fundamental treatment for hereditary hearing loss and inner ear development research.

Original Paper: Takeda H, Hosoya M, Fujioka M, et al. Engraftment of human pluripotent stem cell-derived progenitors in the inner ear of prenatal mice. Scientific Reports. 2018;8(1941). doi:10.1038/s41598-018-20277-5